Engineering TME-activated CD47-specific CAR macrophage via Arg1 promoter for safe and effective solid tumor immunotherapy

Journal for ImmunoTherapy of Cancer | |

Background <p>Chimeric antigen receptor macrophage (CAR-M) therapy has promising therapeutic potential in solid tumors, yet challenges remain in target compatibility and systemic toxicity.</p> Methods <p>In this study, we screened the CD47-scFv sequence of CAR-M as the extracellular structure. We then constructed a classical CD47 CAR-M incorporated the costimulatory domain of the &alpha;1&beta;1 integrin-mediated Fc-gamma receptor I (FcRI) signaling component. Subsequently,…

Topics: blood-cancer, immunotherapy, research