Digital assays are in wide development for biomarker quantification at the single-molecule level, but the common use of surface-pulldown steps limits both analytical sensitivity and throughput. Here, we develop surface-free, wash-free, in-solution assays with a sensitivity slope approaching unity for sequence-specific counting of microRNAs (miRs) relevant to metastatic castration-resistant prostate cancer (mCRPC). These assays are enabled by DNA nanoflowers (DNFs)…
Quantum Dot Encoding for In-Solution Single-Molecule Biomarker Counting in Metastatic Prostate Cancer
medRxiv Oncology | | Kuo, C.-W., Nalla, S., Sarkar, S., Lee, W., Wang, L., Kohli, M., Smith, A. M.
Topics: prostate-cancer, skin-cancer, targeted-therapy, new-technology, research