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DNA Methylation-Based Classification of CNS Tumors: Comparable Performance Between Nanopore and EPIC Technologies

medRxiv Oncology | November 7, 2025 | Alanany, R., Sherif, S., Sanchez, A., Salhab, A., Saadah, H., Ahmed, E., Ozer, E., Maaz, A., Wongsurawat, T., Yousri, N., Raynaud, C., Hendrickx, W.

Background: DNA methylation profiling enables precise classification of pediatric central nervous system (CNS) tumors. Oxford Nanopore Technologies (ONT) offers same-day, single-sample methylation readouts, but its concordance with Illumina EPIC arrays in routine diagnostic tasks remains incompletely defined. Methods: We profiled 23 pediatric tumors (18 CNS, 5 non-CNS) by EPIC (v1/v2; FFPE w/o FF) and ONT (FF). CNS tumors from both…

Topics: oncology

Read the full article at medRxiv Oncology